以 12个苹果砧木基因型为试材 ,建立了扩增酶切片段长度多态性 (AFL P)分析体系。对其分析过程中 DNA提取、双酶切、连接、预扩增、标记和选择性扩增的效果进行了检测。用 P32 M6 4引物构建了供试苹果砧木基因型的 AFL P指纹图谱。该指纹图谱拥有扩增条带 10 5条 ,多态性带 6 7条 (占 6 3.8% ) ,条带清晰可辨 ,扩增信号强 ,无背景干扰。讨论了 AFL P分析的影响因素 A total of 12 apple rootstock genotypes were used as materials to establish an amplified fragment length polymorphism (AFLP) analysis system. The effects of DNA extraction, double digestion, ligation, pre-amplification, labeling and selective amplification were examined during the analysis. The P32 M6 4 primer was used to construct AFL P fingerprinting of apple rootstock genotype. The fingerprinting has 105 amplification bands, 67 polymorphic bands (accounting for 6 3.8%), clear bands, strong amplification signal and no background interference. The influencing factors of AFL P analysis are discussed