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MicroRNAs(miRNAs)在基因表达的内源性调控中起着重要作用。过氧化物酶体增殖物激活受体γ辅激活蛋白1β(peroxisome proliferator-activated receptorγcoactivator 1β,PGC-1β)调控能量消耗和线粒体的生物合成,而miR-378和miR-378*正好位于编码PGC-1β的Ppargc1b基因的第一个内含子中。在心肌细胞中,miR-378:miR-378*发夹结构大量表达。为了更好的研究它们在心肌细胞中的作用,Mallat等采用蛋白质组筛选的方法鉴定miR-378和miR-378*的靶蛋白。他们建立了过表达miR-378和miR-378*的H9c2细胞模型,并且找到了总共87个在任一miRNA作用下表达下调的蛋白。功能注释聚类分析显示miR-378和miR-378*调控的心肌细胞相关通路包括能量代谢(尤其是糖酵解)、细胞骨架(尤其是肌动蛋白丝)和肌肉收
MicroRNAs (miRNAs) play an important role in the endogenous regulation of gene expression. Peroxisome proliferator-activated receptor γ coactivator 1β (PGC-1β) regulates energy expenditure and mitochondrial biosynthesis while miR-378 and miR-378 * are located directly on the site encoding PGC- 1β in the first intron of the Ppargc1b gene. In cardiomyocytes, the miR-378: miR-378 * hairpin structure is abundantly expressed. In order to better study their role in cardiomyocytes, Mallat et al. Used proteomic screening to identify the target proteins of miR-378 and miR-378 *. They established a model of H9c2 cells overexpressing miR-378 and miR-378 * and found a total of 87 proteins whose expression was down-regulated by either miRNA. Functional annotation clustering analysis revealed that cardiomyocyte-associated pathways regulated by miR-378 and miR-378 * include energy metabolism (especially glycolysis), cytoskeleton (especially actin filaments) and muscle contraction