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目的建立HPLC法测定白斑霜中补骨脂素、异补骨脂素含量的方法。方法采用Kromasil 100-5C_(18)色谱柱(250mm×4.6mm,5μm),流动相为乙腈-水(38∶62),流速:0.8ml·min~(-1),柱温:25℃,检测波长:246nm。结果补骨脂素在1.62~32.48ng范围内与峰面积呈良好的线性关系,r=1.0000;异补骨脂素在1.62~32.34ng范围内与峰面积呈良好的线性关系,r=0.9999;补骨脂素平均回收率为99.06%,RSD为1.75%;异补骨脂素平均回收率为96.41%,RSD为1.31%。结论本方法简便、准确,结果稳定,可用于白斑霜中补骨脂素、异补骨脂素的含量测定。
Objective To establish a HPLC method for the determination of psoralen and isopsoralen in the leukoplakia. Methods Kromasil 100-5C 18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase was acetonitrile-water (38:62), the flow rate was 0.8 ml · min -1, the column temperature was 25 ℃, Detection wavelength: 246nm. Results Psoralen showed a good linear relationship with the peak area in the range of 1.62 ~ 32.48ng, r = 1.0000. The isopsoralen showed a good linear relationship with the peak area in the range of 1.62 ~ 32.34ng, r = 0.9999. The average recovery of psoralen was 99.06% with RSD of 1.75%. The average recovery of isopsoralen was 96.41% with a RSD of 1.31%. Conclusion The method is simple, accurate and stable. It can be used to determine the content of psoralen and isopsoralen in the leukoplakia.