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抗人结肠癌单克隆抗体(McAb)MC_3用~(131)Ⅰ标记后在裸鼠人肠癌模型上进行肿瘤定位和放射免疫显像研究。结果显示:体外标记抗体特异性结合率为37.5%,裸鼠体内在48～120h的ECT照相可见在肿瘤部位均有放射性的特异性浓聚,其摄取量随时间延长逐渐增加,肿瘤显影清晰,显像的合适时间为96～120h。而给予非特异性的~(131)Ⅰ-NMIgG后,肿瘤部位未见放射性浓聚,而呈全身均匀性分布。120h肿瘤组织与肝脏及正常肠组织的比值分别为3.61和9.81,肿瘤定位指数为4.26。病理组织学检查显示注射~(131)Ⅰ-MC_3裸鼠肿瘤呈大片坏死,仅局部肿瘤边缘尚存少数完整的肿瘤组织。提示McAbMC_3用于肠癌的诊断和导向治疗可能有良好的前景。
Anti-human colon cancer monoclonal antibody (McAb) MC_3 was labeled with 131I and used for tumor localization and radioimmunoimaging in nude mice. The results showed that the specific binding rate of in vitro labeled antibody was 37.5%. Radioactive specific enrichment was observed at the tumor site in nude mice at 48 to 120 hours of ECT. The uptake was gradually increased with time, and the tumor was clearly visualized. The appropriate time for imaging is 96 to 120 hours. After administration of non-specific 131I-NM IgG, no radioactivity was observed in the tumor site, but the whole body was uniformly distributed. The ratios of tumor tissue to liver and normal intestinal tissue at 120 h were 3.61 and 9.81, respectively, and the tumor localization index was 4.26. Histopathological examination showed that tumors in 131I-MC_3 nude mice showed large necrosis, and only a few intact tumors were present at the edge of local tumors. This suggests that McAbMC_3 may have good prospects for the diagnosis and targeted treatment of colorectal cancer.