论文部分内容阅读
以转Bt基因欧洲黑杨(P.nigra L.)和转CpTI基因毛白杨(Populus tomentosa)叶片饲喂4-5龄美国白蛾（Hyphantria cunea Drury）幼虫，对其体内保护酶系统活性进行测定。结果表明，饲喂两种转基因杨树叶片的幼虫中肠保护酶表现出相似的变化规律，SOD、CAT和POD三种酶的活性在饲喂后数小时内逐渐增加，某一时刻达到最高值，此后突然下降。饲喂转Bt基因杨树叶片的幼虫，其中肠SOD、CAT活性峰值出现在饲喂后的24小时，POD活性峰值出现在饲喂后的12小时；饲喂转CpTI基因杨树叶片的幼虫，其中肠三种保护酶活性高峰出现时间均较前者滞后12小时。本文还比较了饲喂两种转基因叶片不同中毒程度的美国白蛾幼虫体内保护酶活性，发现不论饲喂那种转基因叶片，中毒较轻者其体内保护酶活性显著高于中毒较重者，这种差异在饲喂CpTI叶片的处理株表现尤为明显。
The 4th to 5th instar larvae of Hyphantria cunea Drury were fed on the leaves of transgenic Bt P. nigra and CpTI transgenic Populus tomentosa, . The results showed that the protective enzymes of midgut of larvae fed on two kinds of transgenic poplar leaves showed similar changes. The activity of three enzymes SOD, CAT and POD gradually increased within a few hours after feeding and reached the highest value at a certain moment , Then suddenly dropped. The larvae fed on the leaves of Bt transgenic poplar leaves had the peak of SOD and CAT activities in the midgut at 24 hours after feeding and the peak of POD activity appeared at 12 hours after feeding. The larvae fed poplar leaf with CpTI gene, The intestinal protective enzyme activity peak appeared more time than the former lag 12 hours. In this paper, we also compared the protective enzyme activities of C. furnacalis larvae fed on two transgenic leaves with different degrees of poisoning. The results showed that the protective enzyme activity in vivo was significantly higher than that in severe poisoning Species differences were particularly pronounced in the treated strain fed CpTI leaves.