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目的 观察近交系自发性高胆固醇血症 (NJS)小鼠短暂性脑缺血 (TIA)后的神经行为学和海马细胞改变的情况。方法 采用双侧颈总动脉夹闭后再灌注的方法制备 TIA模型。观察术前及术后小鼠的探究行为、平衡功能 ,统计术后小鼠出现肢体瘫痪的概率和海马神经元的坏死情况 ,并与 ICR小鼠的测定结果相比较。结果 NJS小鼠 TIA后出现肢体瘫痪的概率高 (P =0 .0 2 6 ) ,海马细胞坏死的数量多 ,其中左侧 CA1区、CA3区和齿状核细胞坏死较对照组有统计学差异 (P <0 .0 5 )。肢体瘫痪恢复后探究活动仍较缺血前差 (P <0 .0 5 )。结论 NJS小鼠 TIA后可出现海马神经元坏死和神经行为学改变 ,对其机制的进一步探讨将有助于对部分人群的缺血性脑血管病的神经行为学研究
Objective To observe the changes of neurobehavioral and hippocampal cells after transient cerebral ischemia (TIA) in inbred spontaneous hypercholesterolemia (NJS) mice. Methods TIA model was established by occlusion of bilateral common carotid artery followed by reperfusion. The preoperative and postoperative mice were investigated for their probing behavior and balance function. The probability of limb paralysis and the necrosis of hippocampal neurons were calculated and compared with those of ICR mice. Results The incidence of limb paralysis was significantly higher in NJS mice (P = 0.002 6). The number of necrosis in hippocampus was more in NJS mice. The necrosis of left CA1 zone, CA3 zone and dentate nucleus were statistically significant (P <0. 05). Investigation of limb paralysis recovery is still worse than before ischemia (P <0. 05). Conclusions The neurons in hippocampus necrosis and neurobehavioral changes may occur after TIA in NJS mice. Further study on its mechanism will be helpful for the neurobehavioral studies of ischemic cerebrovascular disease in some people