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本实验以大鼠为实验动物，通过结扎和摘除颈深淋巴结造成脑淋巴引流障碍，观察了不同时间（淋巴引流阻断１、２、３、５、７、１０和１５天）脑结构的变化。大体观察见脑表面苍白、饱满、沟变浅窄、回变宽平，光镜下组织间隙增宽，液体郁滞，神经元变性、坏死，并有大量吞噬细胞浸润，形成“卫星现象”；电镜下神经元肿胀坏死，大胶质细胞肿胀，线粒体等亚细胞结构变化明显。上述结果表明阻断脑淋巴引流对脑结构有明显影响，结构变化构成了“淋巴滞流性脑病”，开拓了脑水肿研究的新领域。本实验成功的建立了大鼠实验性“淋巴性脑水肿”模型。
In this experiment, rats were used as experimental animals, and brain lymphatic drainage was induced by ligation and removal of cervical deep lymph nodes. The changes of brain structure at different time points (lymphatic drainage blocked at 1, 2, 3, 5, 7, 10 and 15 days) . In general, we can see that the brain surface is pale and full, the ditch becomes narrower and narrower, the width becomes wider and wider, the tissue gap broadens under light microscopy, the fluid stagnates, the neurons degenerate and necrosis, and a large number of phagocytic cells infiltrate to form a “satellite phenomenon”. Electron microscope, swelling and necrosis of neurons, large glial swelling, mitochondria and other subcellular structure changes significantly. The above results show that blocking cerebral lymphatic drainage has a significant effect on brain structure, structural changes constitute a “lymphoblastic encephalopathy”, open up new areas of brain edema research. The experimental successful establishment of rat experimental “lymphatic brain edema” model.