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STR（shorttandemrepeat），即短串重复序列，广泛存在于人类基因组中，平均每15kb就存在一个STR位点，为法医个体识别和亲子鉴定提供了丰富的遗传标记[1,2]。复合扩增多个多态片段长度不重叠STR位点，可以节约检材，提高单次PCR扩增的信息量。现将我们对三个STR位点（HHUMCSFI
STR (shorttandemrepeat), which is a short tandem repeat, exists widely in the human genome. An STR locus exists every 15kb on average, providing rich genetic markers for forensic individual identification and paternity testing [1,2]. Multiple amplification of multiple polymorphic fragments without overlapping STR loci can save samples and improve the information of single PCR amplification. Now we have three STR sites (HHUMCSFI