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[目的]探讨不同浓度川芎嗪(TMP)联合丹参酮ⅡA(TanⅡA)对人肺巨细胞癌细胞高转移亚系PG-BE1(PB)、低转移亚系PG-LH7(PL)增殖、黏附及侵袭的影响。[方法]不同浓度的TMP+TanⅡA作用于PB、PL细胞24h、48h、72h后,CCK8检测不同给药组对PB与PL细胞的增殖抑制作用,采用人造基底膜实验与Transwell小室侵袭实验检测细胞黏附及侵袭能力的差异。[结果]TMP+TanⅡA对PB、PL细胞增殖的影响随着浓度增加抑制率增高;随着作用时间的延长,对PB细胞抑制作用增强,对PL细胞抑制作用减弱。TMP+TanⅡA对PB、PL细胞黏附的抑制作用随着药物浓度的增加而增强;在侵袭实验中,TMP+TanⅡA对PB细胞侵袭的抑制率高于PL细胞,其抑制作用也随着药物浓度的增加而增强。[结论]TMP+TanⅡA对人肺巨细胞癌细胞高转移亚系PB、低转移亚系PL具有明显的增殖、黏附及侵袭抑制作用。细胞种系不同,药物的作用时间与浓度对细胞的抑制率也不同。
[Objective] To investigate the effects of TMP combined with TanⅡA on the proliferation, adhesion and invasion of PG-BE1 (PB) and low-metastatic PG-LH7 (PL) cells in human lung giant cell carcinoma cell line, Impact. [Methods] After PBMCs were treated with TMP + TanⅡA at different concentrations for 24, 48, 72 hours, the proliferation of PB and PL cells in different groups were detected by CCK8 assay. The cells were detected by artificial basement membrane assay and Transwell chamber invasion assay Differences in adhesion and invasion ability. [Result] The inhibitory rate of TMP + TanⅡA on the proliferation of PB and PL cells increased with the increase of concentration. With the prolongation of action time, the inhibitory effect on PB cells and the inhibition of PL cells were weakened. The inhibitory effect of TMP + TanⅡA on the adhesion of PB and PL cells was enhanced with the increase of drug concentration. In the invasion experiment, the inhibitory rate of TMP + TanⅡA on PB cell invasion was higher than that of PL cells, Increase and enhance. [Conclusion] TMP + TanⅡA can significantly inhibit the proliferation, adhesion and invasion of high metastatic PB and low metastatic PL of human lung giant cell carcinoma cell line. Different cell lines, the role of drug duration and concentration of the inhibition rate of cells are also different.