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体外分离、培养人子宫内膜基质细胞和腺体细胞，待基质细胞生长融合后，加入孕酮刺激，使其蜕膜化。结果显示，孕酮（ｐｒｏｇｅｓｔｅｒｏｎｅ，Ｐ）组基质细胞胞体变大、变圆，尤以含胎牛血清（ｆｅｔａｌ ｂｏｖｉｎｅ ｓｅｒｕｍ，ＦＢＳ）组显著。用酶免疫分析法检测培养液中催乳素（ｐｒｏ－ｌａｃｔｉｎ，ＰＲＬ）含量发现，培养ｄ１７～２０ＰＲＬ分泌量达到高峰；免疫细胞化学染色可见基质细胞ＰＲＬ免疫反应阳性。在此基础上，将同期培养的腺细胞匀浆液加入到已蜕膜化的基质细胞中，结果发现，在加入腺体匀浆液３ｄ后蜕膜细胞胞体变小，ＰＲＬ分泌量减少，而对照组的ＰＲＬ产量则递增。结果提示，子宫内膜腺细胞对蜕膜反应有一定的抑制作用，其作用方式和机制有待于进一步探讨。
In vitro isolation and culture of human endometrial stromal cells and glandular cells, stromal cells after growth and fusion, adding progesterone stimulation, to decidualization. The results showed that the progesterone (P) group had larger and more rounded cells, especially in the fetal bovine serum (FBS) group. The content of prolactin (PRL) in culture medium was detected by enzyme immunoassay, and the peak of secretion of d17 ~ 20PRL reached its peak. Immunohistochemical staining showed that PRL immunoreactivity of stromal cells was positive. On this basis, the same period of gland cell homogenate was added to the decidualized stromal cells, and found that, after adding glandular homogenate fluid 3d decidual cell body becomes smaller, PRL secretion decreased, while the control group PRL production is increasing. The results suggest that endometrial gland cells have a certain inhibitory effect on decidual response, and its mode of action and mechanism needs further study.