探究卵泡液的成份与卵母细胞成熟及活力之间的关系,结果令人失望;卵泡液中的类固醇激素、蛋白激素与卵母细胞成熟抑制物等成份,因变化太大而在体外受精(IVF)中不能有预测的价值。本研究是应用培养基中再加IVF时所抽取的人卵泡液对单细胞鼠胚作体外培养,观察鼠胚发育结果,以确定在人卵泡液中可能存在的有关影响卵母细胞受精与胚胎存活力的物质。在实验一中,组一用HTF培养基加入来自产生受精胚胎的卵泡液按2%、5%、10%与15%(V/V)浓度培养单细胞实验鼠胚;在组二中用 Explore the relationship between follicular fluid composition and oocyte maturation and vitality, the results disappointing; follicular fluid steroids, protein hormones and oocyte maturation inhibitors and other ingredients, due to changes in too much and in vitro fertilization ( IVF) can not predict the value. This study is the application of medium plus IVF extracted human follicular fluid of single-cell mouse embryos in vitro culture to observe the results of the development of embryos in order to determine the human follicular fluid may affect the oocyte fertilization and embryos Vital substance. In Experiment 1, single-cell experimental mouse embryos were cultured in HTF medium supplemented with 2%, 5%, 10% and 15% (V / V) follicular fluid from fertilized embryos;