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研究了在甲基弯菌(Methylosinustrichosporium)IMV3011整细胞催化甲烷制甲醇的反应过程中,菌体浓度、阻断剂乙二胺四乙酸(ethylenediaminetetraaceticacid,简称EDTA)浓度、外源性电子给体、混合气组分及压力与甲醇积累的关系.批式反应的实验结果表明,在菌体浓度为7.4mg/mL时,以2mmol/LEDTA作阻断剂效果最好;作为电子给体,甲酸钠(20mmol/L)的效果优于琥珀酸钠(40mmol/L),使用前者时的甲醇积累量是用后者时的2.8倍左右;当甲烷与空气的体积比为1∶1.7时,转化率为6.0%,甲醇积累量最大;压力选用0.16MPa.连续反应中,于培养基中无铜离子培养的细胞与有铜离子的相比,持续时间长,甲醇积累高(最大产量达374μmol),前者是后者的2倍.
The effects of cell concentration, ethylenediaminetetraaceticacid (EDTA) concentration, exogenous electron donor, mixed with methanol on the reaction of methanol from methane cell of Methylosinustrichosporium IMV3011 were studied. Gas composition and pressure and methanol accumulation.The experimental results of batch reaction showed that 2mmol / LEDTA was the best blocking agent when the cell concentration was 7.4mg / mL. As the electron donor, sodium formate (20mmol / L) was better than that of sodium succinate (40 mmol / L), the former methanol accumulation was about 2.8 times of that of the latter; when the volume ratio of methane to air was 1: 1.7, the conversion was 6.0 %, The maximum amount of methanol accumulation; pressure selection 0.16MPa. Continuous reaction, in the medium without copper ion-cultured cells compared with copper ions, long duration, high methanol accumulation (maximum yield of 374μmol), the former is The latter two times.