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目的研究双歧杆菌在预防新生鼠坏死性小肠结肠炎中应用剂量的问题,为临床合理用药提供理论依据。方法选取新生SD大鼠40只,随机分为5组:A,正常对照组;B,NEC模型组;C,NEC模型组,每日给予0.4mg/g的酪酸梭菌二联活菌散(主要有效成分为双歧杆菌)灌胃;D,NEC模型组,每日给予4mg/g酪酸梭菌二联活菌散灌胃;E,NEC模型组,每日给予20mg/g酪酸梭菌二联活菌散灌胃。每日记录体重;3d后禁食1d,处死所有新生鼠,取血检测TNF-α并对比;HE染色法观察肠道组织。结果体重变化均值为A>D>E>C>B,除B与C间,D与E间差异无统计学意义,其余组两两对比差异均有统计学意义。HE染色评分均值为B>D>C>E>A,仅A与其他各组间差异有统计学意义。TNF-α均值为B>C>D>E>A,除B与C间,D与E间差异无统计学意义,其余组间均有统计学意义。综合分析,D、E的炎症程度低于B、C。结论酪酸梭菌二联活菌散在新生鼠NEC中应用的最佳预防剂量可能为4~20mg/g,相当于双歧杆菌4.0×10~3~20×10~3 CFU。
Objective To study the application of Bifidobacterium in the prevention of neonatal murine enterocolitis and to provide a theoretical basis for clinical rational drug use. Methods Forty neonatal SD rats were randomly divided into five groups: A, normal control group, B, NEC model group, C, NEC model group, 0.4 mg / The main active ingredient is bifidobacteria) gavage; D, NEC model group, given daily 4mg / g Clostridium butyricum Clostridium perfusion stomach; E, NEC model group, given daily 20mg / g Clostridium butyricum Live bacteria sprinkle stomach. The body weight was recorded daily; after fasting for 1 day, all newborn rats were sacrificed for 1 day, and the levels of TNF-α in blood were measured and compared; the intestinal tissues were observed by HE staining. Results The mean of body weight change was A> D> E> C> B. There was no significant difference between D and E except B and C, and the difference between the other two groups was statistically significant. The mean value of HE staining was B> D> C> E> A, but there was only significant difference between A and other groups. The mean value of TNF-α was B> C> D> E> A. There was no significant difference between B and C except D and E, and the other groups had statistical significance. Comprehensive analysis, D, E less than the degree of inflammation B, C. Conclusion The optimal dose of Clostridium butyricum in the newborn rat NEC may be 4 ~ 20mg / g, which is equivalent to 4.0 × 10 ~ 3 ~ 20 × 10 ~ 3 CFU of Bifidobacterium.