论文部分内容阅读
目的探讨油橄榄叶提取物(OLE)对海洛因依赖小鼠认知和氧化损伤及细胞凋亡的影响。方法 150只小鼠皮下注射海洛因(10 mg/kg)40 d,再分别给予不同剂量的(0、50、100、200 mg/kg)的OLE进行皮下注射治疗,用Morris水迷宫观察空间学习记忆能力的变化,用透射电子显微镜观察海马细胞结构的改变,用比色法检测小鼠海马超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化酶(GPx)、还原型谷胱甘肽(GSH)的活性及丙二醛(MDA)的含量,免疫组织化学和Western blotting检测海马Bax蛋白和Caspase-3的表达。结果模型组小鼠海马神经元核膜不规则,细胞器结构异常或空泡化,小鼠逃避潜伏期和逃避的距离高于正常组,在目标象限探索的时间及游泳距离占总距离的百分比低于正常组(P<0.01),海马组织SOD、CAT、GPx、GSH活性低于正常组,MDA含量高于正常组(P<0.01),海马组织Bax蛋白和caspase-3的表达高于正常组(P<0.01)。OLE治疗组小鼠海马神经元损害程度减轻,小鼠逃避潜伏期和逃避的距离缩短,在目标象限探索的时间及游泳距离占总距离的百分比延长,海马组织SOD、CAT、GPx、GSH活性又升高,MDA含量及Bax蛋白和Caspase-3的表达又显著降低(P<0.05或P<0.01)。结论 OLE对海洛因诱导的脑损伤的神经保护与其具有抗氧化能力。抑制细胞凋亡的特性有一定的关系。
Objective To investigate the effects of Olea europiae leaf extract (OLE) on cognition, oxidative damage and apoptosis in heroin dependent mice. Methods 150 mice were subcutaneously injected with heroin (10 mg / kg) for 40 days. OLE (0, 50, 100 and 200 mg / kg) were given subcutaneously at different doses. Morris water maze was used to observe spatial learning and memory The changes of hippocampal cell structure were observed by transmission electron microscopy. The contents of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) , Glutathione (GSH) and malondialdehyde (MDA) in hippocampus were detected by immunohistochemistry and Western blotting. Results In model group, the nuclear membrane of hippocampal neurons were irregular or the organelles were abnormally or vacuolar. The escape latency and escape distance of mice in model group were higher than those in normal group. The time of exploration in target quadrant and the percentage of swimming distance in total distance were lower than The activity of SOD, CAT, GPx and GSH in hippocampus was lower than that in normal group (P <0.01), and the content of MDA in hippocampus was higher than that in normal group (P <0.01). The expression of Bax protein and caspase- P <0.01). In OLE treatment group, the damage of hippocampal neurons was lessened, the escape latency and escape distance of mice were shortened, and the time of exploration in the target quadrant and the percentage of swimming distance in total distance were prolonged. The activities of SOD, CAT, GPx and GSH in hippocampus increased again High, MDA content, Bax protein and Caspase-3 expression were significantly lower (P <0.05 or P <0.01). Conclusion The neuroprotection of OLE on heroin-induced brain injury has its antioxidant capacity. Inhibit the characteristics of apoptosis have a certain relationship.