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目的探讨P糖蛋白在喉鳞癌组织中表达与肿瘤细胞的增殖、凋亡活性,及其临床病理特征与患者预后的关系。方法应用免疫组化技术检测86例患者喉鳞癌组织中P糖蛋白、Ki-67的表达,TUNEL法测定喉鳞癌细胞凋亡率。结果 P糖蛋白在喉鳞癌组织中的阳性表达率为39.5%(34/86),与喉鳞癌的临床分期、组织学分级、淋巴结转移及患者术后生存率有关(均P<0.05);P糖蛋白表达阳性组的肿瘤细胞凋亡率比阴性组低(P<0.05),而增殖率差异无统计学意义(P>0.05)。结论 P糖蛋白可能对喉鳞癌细胞的凋亡活性具有一定抑制作用;P糖蛋白阳性表达率对于评估喉鳞癌病程进展及预后具有一定价值。
Objective To investigate the relationship between the expression of P-glycoprotein and the proliferation and apoptosis of tumor cells in laryngeal squamous cell carcinoma and its clinicopathological features and prognosis. Methods The expression of P-glycoprotein and Ki-67 in 86 cases of laryngeal squamous cell carcinoma was detected by immunohistochemistry. The apoptosis rate of laryngeal squamous cell carcinoma was detected by TUNEL. Results The positive expression rate of P-glycoprotein in laryngeal squamous cell carcinoma was 39.5% (34/86), which was related to the clinical stage, histological grade, lymph node metastasis and postoperative survival rate of laryngeal squamous cell carcinoma (all P <0.05) The apoptosis rate of tumor cells in positive group with P-glycoprotein expression was lower than that in negative group (P <0.05), but there was no significant difference in proliferation rate (P> 0.05). Conclusions P-glycoprotein may inhibit the apoptosis of laryngeal squamous cell carcinoma cells. The positive rate of P-glycoprotein may be of value in assessing the progression and prognosis of laryngeal squamous cell carcinoma.