【摘 要】
：
The effects of temperature, initial pH, salinity of culture medium, and carbon and nitrogen sources on growth and docosahexaenoic acid (C22: 6 n-3, DHA) product
【机 构】
：
College of Marine Life Sciences,College of Life Sciences
论文部分内容阅读
The effects of temperature, initial pH, salinity of culture medium, and carbon and nitrogen sources on growth and docosahexaenoic acid (C22: 6 n-3, DHA) production from Schizochytrium limacinum OUC88 were investigated in the present study. The results revealed that the optimal temperature, initial pH and salinity level of the medium for DHA production were 23 ℃, 7.0 and 18,respectively. Glucose was proved the best carbon source for the growth and DHA production from S. limacinum. Among the nitrogen sources tested, soybean cake hydrolysate, a cheap by-product, was found to be effective for the accumulation of DHA in S. limacinum cells. In addition, increasing the concentration of carbon sources in the medium caused a significant increase in cell biomass;however, accumulation of DHA in cells was mainly stimulated by the ratio of C/N in the medium. Under the optimal culture conditions, the maximum DHA yield achieved in flasks was 4.08 g L-1 after 5 d of cultivation.
其他文献
为了提高高职高专非英语专业学生英语学习的效率和质量,本文运用实证研究的方法,利用Oxford的学习策略问卷及spss15.0软件对武汉商业服务学院非英语专业学生英语学习策略使用
通过分析交通事件条件下当事车辆瞬时速度的变化特征,设计了1种基于GPS的高速公路交通事件自动检测算法,并对采样时间间隔的确定、检测阈值的确定、持续性检测时间的设定与报警机制设计等方法进行了研究,分别利用软件模拟数据和实测模拟数据对算法的有效性与可移植性进行了检验。试验结果表明该算法对于交通事件具有良好的检测性能。
目的 探讨生长激素治疗小儿全身炎症反应综合征(SIRS)的临床效果.方法 参照第二届世界儿科危重医学大会修订的SIRS诊断标准,观察我院儿科2003年1月～2005年12月收治的SIRS患儿2
为了解沾化凹陷罗家地区油气运聚特征,对由高渗砂体的输导层、断层、不整合面和裂缝组合而成的输导体系进行了研究,发现罗家地区广泛发育断裂、高渗砂体、不整合面和裂缝多种
The Elbe region is representative of humid to semi-humid landscapes in Central Europe,where water availability during the summer season is the limiting factor f
Current glacier recession under the global warming has aroused world-wide attention.Initiated from 1958,the observations show that over the past 50 years,the gl
;主要论述了网络环境下建立地图服务平台的设计思路与结构设计.在此基础上对矢量地图数据基本流程做了详细的论述,其中主要包括数据获取、数据压缩、数据传输、数据表达与空
鉴于脉动真空灭菌器的作用原理,我们对带筛孔金属容器在实施脉动真空灭菌时,打开和关闭筛孔对灭菌效果的影响进行了探讨如下。1材料和方法1.1灭菌器及灭菌参数采用山东新华医
解析交会测量包括测角、测边及边角全测的交会测量.并分析其观测精度及误差,和各自利弊及应用范围.随着光电测距仪器的日益完善和不断普及,测边交会以其显著的优越性弥补了测
本文阐述了机载激光雷达测量原理与组成:建立了激光雷达数据处理的流程图,详述了数据处理的方法:利用EarthData公司提供的试验数据,对激光点云进行自动+人工的滤波处理,将DSM