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目的探讨表没食子儿茶素没食子酸酯[(-)-epigallocatechin-3-gallate,EGCG]联合顺铂(Cisplatin,DDP)对小细胞肺癌细胞株H446的细胞毒作用及其机制。方法采用MTT法检测EGCG及DDP影响H446细胞增殖的量效和时效关系。采用流式细胞仪检测不同浓度EGCG、DDP及二者联合作用于H446细胞对细胞周期及凋亡的影响。结果 EGCG和DDP均可抑制H446细胞增殖,该增殖抑制作用呈浓度及时间依赖模式。不同浓度的EGCG(80、160μmol/L)与DDP(1.0μg/ml)联合应用时,可使H446细胞阻滞于G2/M期。EGCG可诱导H446细胞凋亡,并能增强DDP诱导H446细胞凋亡的作用。结论 EGCG能够抑制H446细胞增殖,诱导H446细胞凋亡,增强DDP诱导H446细胞凋亡的作用,其机制可能与G2/M期阻滞有关。
Objective To investigate the cytotoxicity of epigallocatechin-3-gallate (EGCG) and cisplatin (CDP) on small cell lung cancer cell line H446 and its mechanism. Methods MTT assay was used to detect the effect of EGCG and DDP on the proliferation of H446 cells. Flow Cytometry (FCM) was used to detect the effects of different concentrations of EGCG and DDP on cell cycle and apoptosis in H446 cells. Results Both EGCG and DDP could inhibit the proliferation of H446 cells in a time-and concentration-dependent manner. Different concentrations of EGCG (80,160μmol / L) combined with DDP (1.0μg / ml) could arrest H446 cells in G2 / M phase. EGCG can induce apoptosis in H446 cells and enhance the apoptosis induced by DDP in H446 cells. Conclusion EGCG can inhibit the proliferation of H446 cells, induce the apoptosis of H446 cells and enhance the apoptosis of H446 cells induced by DDP. The mechanism may be related to the G2 / M arrest.