论文部分内容阅读
目的 :研究并比较针刺心肌打孔 (TNP)与激光心肌打孔 (TMLR)对犬急性心肌缺血模型心肌缺血面积、心肌坏死面积及血管新生的影响 ,探讨TNP心肌血运重建术的可行性。方法 :健康杂种犬 12只 ,随机等分为单纯心肌缺血 (SMI)组、TNP组和TMLR组 ,结扎左前降支冠状动脉造成急性心肌缺血后 ,分别进行针刺(TNP组 )和激光 (TMLR组 )打孔。 2周后 ,采用伊文蓝 四氮唑双重染色法测定心肌缺血和心肌坏死面积 ,并通过免疫组化Ⅷ因子抗原染色法观察心肌血管密度。结果 :TNP组和TMLR组术后与SMI组相比心肌缺血面积明显缩小 ,心肌血管密度明显增加 ,但前两组之间比较差异无显著性意义 (P >0 .0 5 ) ;TNP组和TMLR组术后心肌坏死面积相比较亦无显著差异。结论 :TNP能获得TMLR相同的近期效果。在心肌血运重建术中 ,TNP不仅简单易行 ,而且经济有效
Objective: To study the effects of percutaneous puncturing (TNP) and laser drilling of myocardium (TMLR) on myocardial ischemic area, myocardial necrosis area and angiogenesis in acute myocardial ischemia model in dogs, feasibility. Methods: Twelve healthy mongrel dogs were randomly divided into three groups: pure myocardial ischemia (SMI) group, TNP group and TMLR group. Acute myocardial ischemia was induced by ligation of the left anterior descending coronary artery and acupuncture (TNP group) and laser (TMLR group) perforated. Two weeks later, the area of myocardial ischemia and myocardial necrosis was measured by IV tetrazolium double staining, and the myocardial vascular density was observed by immunohistochemical Ⅷ factor antigen staining. Results: Compared with the SMI group, the area of myocardial ischemia in the TNP group and the TMLR group were significantly reduced, and the myocardial vascular density was significantly increased. However, there was no significant difference between the two groups (P> 0.05) There was no significant difference in myocardial necrosis area between TMLR group and postoperative. Conclusion: TNP can achieve the same immediate effect of TMLR. In revascularization, TNP is not only simple and cost effective