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目的以斑点杂交法及PCR定量检测法评价干扰素抗HBV的疗效。方法90例慢性乙肝患者干扰素治疗前后均同时用两种方法检测血清HBVDNA。结果HBVDNA定量在总体上呈下降趋势。治疗后 2个月末与 4个月末其病毒拷贝数分别为 5 .83± 1.0 5 ,5 .75± 1.2 1,与治疗前 6 .74± 1.6 8相比差异均有显著性 (P <0 .0 5 )。HBVDNA定量PCR较斑点杂交更能反映干扰素治疗过程中患者外围血内病毒拷贝数的增长。结论两种方法均能灵敏地反映干扰素抗HBV的疗效 ,但在实际中定量PCR更灵敏。
Objective To evaluate the efficacy of interferon anti-HBV by dot blot hybridization and PCR quantitative assay. Methods 90 cases of chronic hepatitis B patients before and after treatment with interferon both at the same time using two methods to detect serum HBVDNA. Results HBVDNA quantification showed a general downward trend. The virus copy numbers at the end of 2 months and the end of 4 months after treatment were respectively 5.83 ± 1.05 and 5.75 ± 1.2 1, which were significantly different from those before treatment (6. 74 ± 1.6 8, P <0. 0 5). HBVDNA quantitative PCR better reflects the increase of virus copy number in peripheral blood of patients during interferon treatment than that of dot blot. Conclusion Both methods can sensitively reflect the efficacy of interferon against HBV, but in practice, quantitative PCR is more sensitive.