论文部分内容阅读
目的探讨转化生长因子-β(TGF-β)对体外培养的人角膜基质细胞增殖的影响。方法体外培养的传2～3代人角膜基质细胞,分别加入0.01,0.1,1,5ng/ml的TGF-β,采用MTT法研究TGF-β对人角膜基质细胞增殖的影响。结果TGF-β以剂量依赖方式促进人角膜基质细胞增殖,其作用在第2天达到高峰。结论TGF-β能明显促进人角膜基质细胞增殖,因而可能成为促进角膜溃疡愈合的新方法。
Objective To investigate the effect of transforming growth factor-β (TGF-β) on the proliferation of human corneal stromal cells cultured in vitro. Methods Cultured corneal stromal cells from 2 to 3 passages were cultured in vitro. TGF-β at 0.01, 0.1, 1 and 5 ng / ml respectively were added into the culture medium. The effects of TGF-β on the proliferation of human corneal stromal cells were investigated by MTT assay. Results TGF-β promoted the proliferation of human corneal stromal cells in a dose-dependent manner, and its effect peaked on the 2nd day. Conclusion TGF-β can significantly promote human corneal stromal cell proliferation, which may be a new method to promote the healing of corneal ulcer.