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目的:优选川乌药材中总生物碱的最佳提取工艺。方法:采用L9(34)正交法,以提取物得率和乌头碱、次乌头碱和新乌头碱的含量为考察指标,并用RP-HPLC法测定三种生物碱的含量,流动相为甲醇-水-氯仿-二乙胺(70∶30∶2∶0.1)。结果:筛选出的最佳提取工艺为药材用氨试液浸润,加入15倍乙醚溶剂,冷浸24h。结论:该实验提取工艺合理、科学。
Objective: To optimize the extraction of total alkaloids from Radix Chuanxiong. Methods: The content of aconitine, hypaconitine and mesaconitine were determined by L9 (34) orthogonal test. The contents of three alkaloids were determined by RP-HPLC The phase was methanol-water-chloroform-diethyl amine (70:30: 2: 0.1). Results: The best extraction process for the screening of herbs with ammonia solution infiltration, adding 15 times the ether solvent, cold soak 24h. Conclusion: The experimental extraction process is reasonable and scientific.