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目的在体外比较大黄酸和大黄素作用于人肾小管上皮细胞(HK-2)的毒性表现和差异,并初步探讨其肾毒性机制。方法采用MTT法观察细胞生长抑制作用;透射电镜观察细胞内超微结构变化;流式细胞仪观察细胞凋亡情况;检测乳酸脱氢酶(LDH)和N-乙酰-β-D-氨基葡萄糖苷酶(NAG)释放水平。结果大黄酸和大黄素均可明显抑制HK-2细胞增殖;电镜观察均可见细胞核不规则,出现了染色质的浓缩和边集;流式细胞仪观察大黄素和大黄酸均能诱导细胞凋亡;大黄素组LDH和NAG释放水平均高于大黄酸组。结论大黄酸和大黄素均能诱导HK-2细胞凋亡,具有明显的细胞毒性作用,并且大黄素对HK-2细胞的毒性作用强于大黄酸。
Objective To compare the toxic manifestations and differences of rhein and emodin in human renal tubular epithelial cells (HK-2) in vitro and to explore their nephrotoxicity mechanisms. Methods The cell growth inhibition was observed by MTT method. The ultrastructure of the cells was observed by transmission electron microscopy. The apoptosis of cells was observed by flow cytometry. The activities of lactate dehydrogenase (LDH) and N-acetyl-β-D-glucosaminide Enzyme (NAG) release levels. Results Both rhein and emodin significantly inhibited the proliferation of HK-2 cells. The electron microscope showed irregular nuclei and chromatin condensation and edge sets. Emodin and rhein could both induce cell apoptosis by flow cytometry The release of LDH and NAG in emodin group was higher than that in rhein group. Conclusion Both rhein and emodin can induce the apoptosis of HK-2 cells and have obvious cytotoxicity. Emodin is more toxic to rheumatoid arthritis HK-2 cells than rhein.