顺铂(DDP)是治疗睾丸、卵巢及头颈部肿瘤的最有效药物之一,但会因产生耐药性而导致治疗失败,产生耐药性的机理尚不明了。能引起对DDP耐药的生化改变有:(1)细胞内DDP蓄积减少;(2)谷胱甘肽水平或谷胱甘肽-S-转移酶活性升高;(3)细胞内金属硫蛋白水平升高;(4)DNA修复活动增强。许多研究证明药物蓄积与敏感性之间有一定联系,在大多数病例中耐药亚株比药物敏感株细胞中蓄积的DDP少。尽管这可以说明耐药表型的重要 Cisplatin (DDP) is one of the most effective drugs for the treatment of testicular, ovarian and head and neck cancers, but it can lead to treatment failure due to drug resistance. The mechanism of drug resistance is not known. Biochemical changes that can cause resistance to DDP include: (1) decreased accumulation of DDP in cells; (2) increased glutathione levels or glutathione-S-transferase activity; (3) intracellular metallothioneins Increased levels; (4) Increased DNA repair activity. Many studies have shown that there is a certain relationship between drug accumulation and sensitivity. In most cases, drug-resistant sub-strains have less accumulated DDP than drug-sensitive cells. Although this can explain the importance of drug resistant phenotypes