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目的:建立了人参中11种人参皂苷同时检测的超高效液相色谱分析方法。方法:采用Waters Ac-quity UPLC BEH C18色谱柱(1.7μm,50 mm×2.1 mm)、乙腈-水流动相(流速0.5 ml/min)柱温30℃、检测波长:203 nm的色谱条件,同时分离测定人参皂苷R1、Rg1、Re、Rf、Rh1、Rg2、Rb1、Rc、Rb2、Rd、Rg3。样品以水饱和正丁醇为提取溶剂,经大孔吸附树脂柱除去基质干扰,取2μl注入UPLC。结果:11种人参皂苷在10 mg/L～100 mg/L范围内呈良好的线性关系,方法定量限为0.01%,加标回收率在90.9%～101.8%,所测定的RSD在1.89%～4.23%之间。结论:方法灵敏、快速,结果准确可靠。
Objective: To establish a method for simultaneous determination of 11 ginsenosides in ginseng by ultra performance liquid chromatography. METHODS: Waters Ac-quity UPLC BEH C18 column (1.7 μm, 50 mm × 2.1 mm) and acetonitrile-water mobile phase (flow rate 0.5 ml / min) were used to determine the chromatographic conditions at a wavelength of 203 nm Ginsenosides R1, Rg1, Re, Rf, Rh1, Rg2, Rb1, Rc, Rb2, Rd, Rg3 are separately measured. Samples were saturated water butanol as the extraction solvent, the macroporous resin column to remove matrix interference, and 2μl into the UPLC. Results: The 11 kinds of ginsenosides showed a good linear relationship in the range of 10 mg / L ~ 100 mg / L with the limit of quantification of 0.01% and the spiked recoveries of 90.11% ~ 101.8%. The measured RSDs ranged from 1.89% 4.23% between. Conclusion: The method is sensitive and fast, the result is accurate and reliable.