本实验采用24孔塑料细胞培养板培养新生大鼠心肌细胞,生长48 h后再置于缺氧、缺糖环境培养,观察了水飞蓟宾对缺氧、缺糖的心肌细胞的作用。实验结果表明,加水飞蓟宾后再进行缺氧、缺糖培养的心肌细胞受损减轻,其受损后释放的乳酸脱氢酶减少,较对照组相差非常显著。在本实验中,水飞蓟宾不仅可以增加心肌细胞对缺氧、缺糖的耐受性,而且可以部分对抗异丙肾上腺素对缺氧、缺糖心肌细胞的损伤作用。 In this study, 24-well plastic cell culture plate cultured neonatal rat cardiomyocytes, 48 ​​h after growth and then placed in hypoxia, hypoglycemic environment, the effect of silybin in hypoxic and glucose-deficient cardiomyocytes. The experimental results showed that myocardial cells damaged by hypoxia and glucose deprivation were reduced after silybin was added, and the lactate dehydrogenase released after the injury was reduced, which was significantly different from the control group. In this experiment, silybin can not only increase the tolerance of myocardial cells to hypoxia and glucose deprivation, but also partially antagonize the injury of isoproterenol to hypoxia and hypoglycemic cardiomyocytes.