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本研究运用免疫组化SP法,对92例手术切除胃癌组织及所属淋巴结和2 8例癌旁正常黏膜组织石蜡标本进行了抑癌基因p16、p5 3突变型、原癌基因C erbB 2蛋白进行了检测,就3个基因对胃癌淋巴转移的作用进行了分析。报告如下。1 资料和方法92例胃癌标本及所属淋巴结,取自1997～2 0 0
In this study, immunohistochemical SP method was used to detect the tumor suppressor gene p16, p53 mutations and the oncogene C erbB 2 protein in 92 paraffin-embedded gastric cancer tissues and their associated lymph nodes and 28 paracancerous normal paraffin tissues. Assays were performed to analyze the role of the three genes in the lymph node metastasis of gastric cancer. The report is as follows. 1 Materials and Methods 92 cases of gastric cancer specimens and their lymph nodes, taken from 1997 to 2000