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目的探讨叶黄素防护视网膜蓝光损伤的相关机制。方法将大鼠按体重随机分为6组:正常对照组、模型对照组、溶剂对照组以及叶黄素低、中、高剂量组。叶黄素低、中、高剂量组分别注射0.5、1.0和2.0mg/ml的叶黄素,溶剂对照组注射由生理盐水和Tween80以9:1混合的溶剂,注射剂量均为5μl。暗适应24h,利用蓝光光损伤仪建立大鼠视网膜光损伤模型,光暴露时间为2h。光暴露结束后暗适应72h,处死取视网膜测定氧化应激指标、神经元型一氧化氮合酶(nNOS)和c-fos蛋白的表达情况。结果与模型对照组及溶剂对照组相比,叶黄素剂量组大鼠视网膜中丙二醛(MDA)含量显著减少(P<0.05),而SOD和GSH-Px的活性在各组间差异无显著性。叶黄素剂量组大鼠视网膜中c-fos蛋白的表达量低于溶剂对照组和模型对照组(P<0.05),而nNOS的表达量与其他组相比差异无显著性(P>0.05)。结论叶黄素可能通过淬灭氧自由基,抑制脂质过氧化和c-fos基因的表达发挥其保护作用。
Objective To investigate the mechanism of lutein protection against blue light damage in retina. Methods The rats were randomly divided into 6 groups according to body weight: normal control group, model control group, solvent control group and lutein low, medium and high dose groups. The lutein low, medium and high dose groups were injected with 0.5, 1.0 and 2.0 mg / ml of lutein, the solvent control group injected by saline and Tween80 mixed solvent 9: 1, the injection dose was 5μl. Dark adapted 24h, the use of blue light damage instrument rat retinal light damage model, light exposure time of 2h. After dark exposure for 72h, the retina was sacrificed to measure the expression of oxidative stress index, neuronal nitric oxide synthase (nNOS) and c-fos protein. Results Compared with the model control group and the solvent control group, the content of malondialdehyde (MDA) in the retina of the lutein group was significantly decreased (P <0.05), while the activity of SOD and GSH-Px was no difference between the groups Significance. The expression of c-fos protein in retina of lutein group was lower than that of solvent control group and model control group (P <0.05), while the expression of nNOS was not significantly different from other groups (P> 0.05) . Conclusion Lutein may exert its protective effect by quenching oxygen free radicals, inhibiting lipid peroxidation and c-fos gene expression.