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目的探讨聚丙烯酰胺凝胶的最佳聚合条件。方法体外建立模拟循环模型,将30%丙烯酰胺贮存液分别配制成浓度为6%、7%、8%、9%的聚丙烯酰胺凝胶试验液,在37℃恒温环境下,寻找凝胶聚合最适浓度、四甲基乙二胺(TEMED)∶10%过硫酸铵(AP)值及最适推注速度。结果 37℃条件下,TEMED∶AP分别为1∶5、1∶10、1∶15,凝胶浓度为6%时的聚合时间分别为(43.3±1.9)、(117.6±3.0)、(433.0±17.0)s,凝胶浓度为7%时的聚合时间分别为(30.2±1.4)、(33.8±1.9)、(47.0±2.0)s,凝胶浓度为8%时的聚合时间分别为(20.3±1.2)、(22.4±1.4)、(25.0±1.1)s,凝胶浓度为9%时的聚合时间分别为(18.3±1.2)、(26.1±1.3)、(29.5±1.3)s。凝胶聚合时间<45 s、45~50 s、>50 s时聚合有效率分别为6.67%、90%、10%,两两比较,P均<0.01。凝胶溶液最适推注速度为0.1 mL/s。结论聚丙烯酰胺凝胶的最佳聚合条件为37℃环境下,利用30%丙烯酰胺贮存液配制成7%丙烯酰胺凝胶溶液,取TEMED∶AP=1∶15,推注速度为0.1 mL/s。
Objective To investigate the best polymerization conditions of polyacrylamide gel. Methods A simulated circulation model was established in vitro. The 30% acrylamide storage solutions were respectively prepared into polyacrylamide gel test solution with concentration of 6%, 7%, 8% and 9%. Under the constant temperature environment of 37 ℃, Optimum concentration, tetramethylethylenediamine (TEMED): 10% ammonium persulfate (AP) value and optimum bolus speed. Results At 37 ℃, the polymerization times of TEMED: AP were 1:5, 1:10 and 1:15, respectively, and the gel time was 43.3 ± 1.9, 117.6 ± 3.0 and 433.0 ± 17.0) s, respectively. The polymerization times at the gel concentration of 7% were (30.2 ± 1.4) and (33.8 ± 1.9) and (47.0 ± 2.0) s respectively. The polymerization times at gel concentration of 8% 1.2, 22.4 ± 1.4 and 25.0 ± 1.1 s respectively. The polymerization time was (18.3 ± 1.2), (26.1 ± 1.3) and (29.5 ± 1.3) s when the gel concentration was 9%. Gel polymerization time <45 s, 45 ~ 50 s,> 50 s polymerization efficiency were 6.67%, 90%, 10%, any pairwise comparison, P all <0.01. The optimal bolting speed of the gel solution was 0.1 mL / s. CONCLUSION Polyacrylamide gel was prepared under the conditions of 37 ℃, 7% acrylamide gel solution with 30% acrylamide solution, TEMED: AP = 1:15, and the injection rate of 0.1 mL / s.