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采用多克隆双抗体夹心ＥＬＩＳＡ法快速检测狂犬病毒抗原含量。结果表明，灵敏度达到１５μｇ／ｍｌ，同时特异性及变异系数均合乎要求。本方法用于精制狂苗制备过程中有效抗原活性组分的检测准确、快速、重复性好，且抗原的ＥＬＩＳＡ效价与ＮＩＨ动物法测定效价具有平行趋势。
Rapid detection of rabies virus antigen content by polyclonal antibody sandwich ELISA. The results showed that the sensitivity of 15μg / ml, both specificity and coefficient of variation were satisfactory. The method has the advantages of accurate, rapid and good repeatability in the detection of active antigen components during the process of preparation of the refined seedlings. The ELISA titer of the antigen and the NIH animal method have a parallel trend.