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目的：探讨抑癌基因P（53）突变与宫颈癌发生发展之间的关系，并为该病的临床检测提供一种分子生物学方法。方法：应用多聚酶链反应-单链构象多态性（PCR－SSCP）分析方法对宫颈癌及慢性宫颈炎组织中P（53）基因5－6外显子的突变进行了检测。结果：35例宫颈癌组织中有2例出现突变，突变率为5．71％；而作为对照的慢性宫颈炎组织中无一例出现突变。结论：宫颈癌的发生发展与抑癌基因P（53）－6外显子的突变有关；此方法可有效地检测抑癌基因P（53）的突变。
Objective: To investigate the relationship between the mutation of tumor suppressor gene P (53) and the development of cervical cancer and to provide a molecular biological method for the clinical detection of this disease. Methods: The mutation of exon 5-6 of P (53) gene in cervical cancer and chronic cervicitis was detected by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). Results: Two of the 35 cases of cervical cancer showed a mutation with a mutation rate of 5.71%. No mutation was found in the control group of chronic cervicitis. Conclusion: The occurrence and development of cervical cancer is related to the mutation of P (53) -6 exon; this method can effectively detect the mutation of tumor suppressor gene P (53).