采用三种方法从感染细胞中分离HSV DNA。同时对用三种方法提取的HSV DNA的量以及与细胞DNA分离程度的差别作了比较。采用其中一种方法对10株流行病学上无关的、分别来自疱疹性角膜炎和口唇疱疹病人的分离病毒株,用限制性核酸内切酶BglⅡ、HindⅢ和BamHⅠ进行了酶切分析与鉴定,BglⅡ和HindⅢ可明显区分HSV的型间差别,BamHⅠ可对10株HSV进行株间的鉴定。 Three methods are used to isolate HSV DNA from infected cells. At the same time, the amount of HSV DNA extracted by three methods and the difference with the degree of DNA isolation were compared. One of these methods was used to isolate and analyze 10 isolates that were epidemiologically unrelated to herpes keratitis and herpes labialis respectively and digested with restriction endonucleases BglII, HindIII and BamHI. Bgl Ⅱ and Hind Ⅲ can clearly distinguish between the type of HSV, BamH Ⅰ 10 strains of HSV can be identified between strains.