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以Poloxamer188为乳化剂 ,乙酸乙酯为有机溶剂 ,采用复乳法制备了胰岛素乳酸 羟基乙酸共聚物 (PLGA)纳米粒。考察了乳化剂和PLGA的浓度、内水相中胰岛素的浓度及pH、溶剂挥发方法和内水相中加入聚乙烯醇 (PVA)等实验各因素对胰岛素PLGA纳米粒包封率的影响。结果表明 ,乳化剂的浓度较高、PLGA的浓度较小、内水相的pH接近胰岛素的pI(5 3)、胰岛素的浓度较低、缩短有机溶剂挥发时间及内水相中加入PVA有利于提高胰岛素的包封率。经实验条件优化后制备的胰岛素PLGA纳米粒平均粒径为 149 6nm ,多分散性系数小于 0 1,包封率提高到 42 8%。
Poloxamer188 was used as emulsifier and ethyl acetate was used as the organic solvent to prepare insulin-lactic glycolic acid (PLGA) nanoparticles by double emulsion method. The effects of various factors such as the concentration of emulsifier and PLGA, the concentration and pH of insulin in the inner aqueous phase, the solvent evaporation method and the addition of polyvinyl alcohol (PVA) in the inner aqueous phase on the encapsulation efficiency of insulin PLGA nanoparticles were investigated. The results showed that the concentration of emulsifier was higher, the concentration of PLGA was smaller, the pH of inner aqueous phase was close to that of insulin (5 3), the concentration of insulin was lower, the evaporation time of organic solvent was shortened, Increase insulin encapsulation efficiency. The average particle size of the prepared PLGA NPs was 149 6nm, the polydispersity index was less than 0.01, and the encapsulation efficiency increased to 42.8%.