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目的探讨CD4+CD25+调节性T细胞(regulatory T cells,Treg)在小鼠自身免疫性卵巢疾病(autoimmune ovarian disease,AOD)发生与发展过程中的变化及意义。方法出生第3天乳鼠摘除胸腺(thymectomized on day 3,D3tx)建立D3tx组和假手术组,免疫组织化学观察小鼠卵巢改变,ELISA检测性激素变化,流式细胞学检测术后小鼠外周血中Treg及T细胞亚群比例的变化。结果胸腺切除后5周卵巢出现炎细胞浸润及卵泡闭锁,血清FSH升高,E2下降。小鼠外周Treg在术后1～3周达到高峰后快速下降,缓慢下降直至术后9周仍高于对照组(P<0.05);AOD发生后,CD3+T细胞明显减低(P<0.001),CD4+T减低(P<0.05),CD8+T无明显统计学差异(P>0.05),CD4+/CD8+T细胞比例有下降趋势(P>0.05),但无统计学意义。结论 Treg参与AOD的发病过程,推测可能在AOD发病初期具有潜在保护作用。
Objective To investigate the changes and significance of CD4 + CD25 + regulatory T cells (Tregs) in the development and progression of autoimmune ovarian disease (AOD) in mice. Methods D3tx group and sham operation group were established by thymectomized on day 3 (D3tx). Ovary changes of mice were observed by immunohistochemical method. Changes of sex hormones were detected by ELISA. Flow cytometry In the proportion of Treg and T cell subsets. Results 5 weeks after thymectomy ovarian inflammatory cell infiltration and follicular atresia, serum FSH increased, E2 decreased. The peripheral Treg of mice decreased rapidly at 1 ~ 3 weeks postoperatively and then slowly decreased until 9 weeks postoperatively (P <0.05). After AOD, CD3 + T cells decreased significantly (P <0.001) , CD4 + T decreased (P <0.05). There was no significant difference in CD8 + T (P> 0.05) and CD4 / CD8 + T cells decreased (P> 0.05) Conclusion Treg is involved in the pathogenesis of AOD, suggesting that Treg may have a potential protective effect in the early stage of AOD.