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目的：应用犬低温体外循环灌注模型，评价在心脏停跳液中添加三七总皂甙（ＰＮＳ）的心肌保护作用。方法：实验犬１８只，雌雄不分，随机分为３组（每组６只）。１组为对照组，用冷钾停跳液，２组为ＰＮＳ（５０ｍｇ／Ｌ）小剂量组，３组为ＰＮＳ（１５０ｍｇ／Ｌ）大剂量组。结果：心功能指标恢复百分数，超氧化物歧化酶和脂质过氧化产物丙二醛生成，肌酸激酶及其同工酶活力３组比较：１组缺血再灌注后心功能恢复较差，超氧化物歧化酶明显下降（Ｐ＜０．０１），丙二醛及心肌酶明显升高（Ｐ＜０．０１），２组、３组则变化不明显（Ｐ＞０．０５）。１组与２组、３组比较有显著差异（Ｐ＜０．０１），２、３组间亦有显著差异（Ｐ＜０．０１）。心肌超微结构的保护１组最差，３组最好。结论：在心脏停跳液中加较小剂量的ＰＮＳ具有良好的心肌保护作用，加大剂量效果更佳。
OBJECTIVE: To evaluate the cardioprotective effect of Panax notoginseng saponins (PNS) in cardioplegia in dogs using a hypothermic perfusion model. METHODS: Eighteen experimental dogs were randomly divided into three groups (n=6 in each group). One group was the control group, which was treated with cold potassium ampholytes, two groups were PNS (50 mg/L) low dose group, and three groups were PNS (150 mg/L) high dose group. RESULTS: Percent recovery of cardiac function parameters, superoxide dismutase and malondialdehyde production of lipid peroxidation products, and creatine kinase and its isoenzyme activity in three groups were compared: one group had poor recovery of cardiac function after ischemia and reperfusion. Superoxide dismutase decreased significantly (P<0.01), malondialdehyde and myocardial enzymes increased significantly (P<0.01), but there was no significant change between groups 2 and 3 (P>0.05). There was a significant difference between group 1 and group 2 and group 3 (P<0.01), and there was also a significant difference between groups 2 and 3 (P<0.01). Myocardial ultrastructure protection group 1 was the worst, and group 3 was the best. Conclusion: The addition of a small dose of PNS in cardioplegia has a good myocardial protective effect, and the effect of increasing the dose is better.