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采用中心组合设计,以菌丝体生物量为考察指标对虫草液体发酵的非营养条件进行优化,同时对菌丝体提取物的免疫功能进行了探讨。研究结果表明:当培养温度为22.64℃、pH为6.18、培养时间为7.36d时,菌丝体产量达到21.73g/L;并发现其水提取物在50～200μg/mL的剂量范围内,能够提高巨噬细胞吞噬能力和促进小鼠脾细胞的增殖,具有一定的免疫调节作用,是一种潜在的具有免疫调节功能的食品资源。
The center combination design was used to optimize the non-nutritive conditions of liquid fermentation of Cordyceps sinensis with mycelial biomass as the indicator, and the immune function of mycelium extracts was also discussed. The results showed that when the culture temperature was 22.64 ℃, the pH was 6.18 and the culture time was 7.36 days, the mycelial yield reached 21.73 g / L. The results showed that the water extract could reach the dose range of 50 ~ 200 μg / mL Improve phagocytosis of macrophages and promote the proliferation of mouse spleen cells, has a certain immunomodulatory effect, is a potential immune regulatory function of food resources.