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目的:观察PPARγ激动剂吡格列酮和拮抗剂GW9662对巨噬细胞脂质蓄积和MMP9 mRNA表达的影响。方法:提取昆明小鼠腹腔巨噬细胞,以oxLDL作为泡沫细胞诱导剂,分组培养24h和72h。油红O染色观察细胞脂质蓄积情况,聚合酶链反应检测细胞MMP9 mRNA表达的变化。结果:在24h点,GW9662能抑制oxLDL诱导的巨噬细胞脂质蓄积;另一方面,在24h和72h两个时间点,吡格列酮能抑制oxLDL诱导的巨噬细胞MMP9 mRNA表达(分别为0.017和0.038)。结论:GW9662可能对动脉粥样硬化斑块病变的巨噬细胞泡沫化过程起拮抗作用,而吡格列酮在维持动脉粥样硬化斑块稳定性方面有潜在作用。
Objective: To observe the effect of PPARγ agonist pioglitazone and antagonist GW9662 on macrophage lipid accumulation and MMP9 mRNA expression. Methods: Kunming mouse peritoneal macrophages were isolated and oxLDL was used as a foam cell inducer. The cells were cultured for 24 h and 72 h. Oily red O staining was used to observe the accumulation of lipid in the cells. The expression of MMP9 mRNA was detected by polymerase chain reaction. Results: At 24h, GW9662 could inhibit oxLDL-induced macrophage lipid accumulation. On the other hand, at 24h and 72h, pioglitazone inhibited oxLDL-induced MMP9 mRNA expression in macrophages (0.017 and 0.038 ). CONCLUSION: GW9662 may antagonize the foaming of macrophages in atherosclerotic lesions, while pioglitazone may have a potential role in maintaining the stability of atherosclerotic plaques.