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目的建立救必应药材中冬青素A的含量测定方法。方法采用反相高效液相色谱法。色谱柱:Dia-monsil C18柱(250 mm×4.6 mm,5μm);流动相:乙腈(A)与0.4%磷酸(B),梯度洗脱;体积流量:1 mL.min-1;检测波长:210 nm;柱温:25℃;进样量:20μL。结果冬青素A在12.5~500μg.mL-1范围内与峰面积呈良好的线性关系(r=0.9998),平均回收率为99.22%,RSD=1.85%(n=6)。结论该方法简便、准确、可靠且分离度好,可用于救必应药材中冬青素A的含量测定。
Objective To establish a method for the determination of hollyin A Methods Using reversed-phase high performance liquid chromatography. Column: Dia-monsil C18 column (250 mm × 4.6 mm, 5 μm); mobile phase: acetonitrile and 0.4% phosphoric acid; gradient elution; 210 nm; column temperature: 25 ℃; injection volume: 20μL. Results The linear relationship between peak area and the area of 12.5 ~ 500μg.mL-1 was obtained for the determination of trace elements (r = 0.9998). The average recoveries were 99.22% and RSD = 1.85% (n = 6). Conclusion The method is simple, accurate, reliable and good resolution and can be used to determine the content of