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从改进现有的血吸虫病免疫诊断以及发展针对日本血吸虫感染的实验性分子疫苗的目标着想,考虑到需要制备克隆化的抗原。目前已采用日本血吸虫(中国株)成虫的mRNA,在载体λgtll 噬菌体以及宿主大肠杆菌y1090这一系统中构建了一个cDNA 表达库,并对该表达库用抗体进行筛选。有关建库的基本步骤描述于后:
With the objective of improving the current immunological diagnosis of schistosomiasis and developing an experimental molecular vaccine against Schistosoma japonicum infection, it is considered necessary to prepare a cloned antigen. At present, the mRNA of adult Schistosoma japonicum (Chinese strain) has been used to construct a cDNA expression library in the vector λgtll phage and the host E. coli y1090 system, and the expression library is screened with antibodies. The basic steps for building a database are described below: