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目的:肺表面活性蛋白A(SP-A)在呼吸道中发挥重要的宿主防御作用。本研究的目的是了解SP-A在变应性鼻炎鼻黏膜和鼻息肉组织中的表达情况,探讨SP-A在上呼吸道炎症反应性疾病中发挥的作用。方法:采用免疫组织化学方法和间接免疫荧光法分别检测11例变应性鼻炎患者鼻黏膜组织(变应性鼻炎组)、15例鼻息肉患者的息肉组织(鼻息肉组)和7例行鼻中隔偏曲矫正术患者的中鼻甲组织(对照组)中SP-A的表达。结果:免疫组织化学方法结果显示变应性鼻炎组及鼻息肉组中SP-A的表达明显高于对照组(P<0.05),变应性鼻炎组及鼻息肉组黏膜下嗜酸粒细胞的数量明显高于对照组(P<0.05),变应性鼻炎组及鼻息肉组中SP-A的表达与黏膜下嗜酸粒细胞的数量均呈正相关(r分别为0.81,0.55);免疫荧光法结果显示SP-A在变应性鼻炎组及鼻息肉组黏膜上皮细胞内的绿色荧光强度高于对照组(P<0.05)。结论:SP-A可能参与了变应性鼻炎与鼻息肉的炎症反应过程。SP-A在上呼吸道炎症性疾病中的表达,为今后寻找新的治疗方法提供理论思路。
PURPOSE: Pulmonary surfactant protein A (SP-A) plays an important host defensive role in the respiratory tract. The purpose of this study is to understand the expression of SP-A in nasal mucosa and nasal polyps of allergic rhinitis, and to explore the role of SP-A in the upper respiratory tract inflammatory disease. Methods: Immunohistochemistry and indirect immunofluorescence were used to detect the nasal mucosa (allergic rhinitis) in 11 patients with allergic rhinitis, the polyp tissue (nasal polyps) in 15 patients with nasal polyps and the nasal polyps SP-A expression in middle turbinate tissue (control group) in patients with skewness correction. Results: The results of immunohistochemistry showed that the expression of SP-A in allergic rhinitis group and nasal polyps group was significantly higher than that in control group (P <0.05), and the percentage of submucosal eosinophils in allergic rhinitis group and nasal polyps group (P <0.05). There was a positive correlation between the expression of SP-A and the number of submucosal eosinophils in allergic rhinitis group and nasal polyps group (r = 0.81,0.55, respectively) The results showed that the green fluorescence intensity of SP-A in mucosal epithelial cells of allergic rhinitis group and nasal polyps group was higher than that of the control group (P <0.05). Conclusion: SP-A may be involved in the inflammatory reaction of allergic rhinitis and nasal polyps. The expression of SP-A in inflammatory diseases of the upper respiratory tract may provide a theoretical basis for finding new treatments in the future.