本文对人外周血中淋巴细胞培养时间进行了研究,在动态观察中外周血的淋巴细胞36小时开始出现分裂相、40小时达第一次高峰,分裂指数为7.2％,分裂细胞全部为M_1期。52小时M_2细胞相达51.3％,M_3细胞相为0.3％。40小时淋巴细胞培养的分裂相10例均值为9.08％,分裂相中的中期为74.0％,全部为M_1细胞相,因而质量完全符合需要,故40小时培养的人外周血淋巴细胞实验法是分析染色体畸变的较好的方法。 In this paper, the culture time of lymphocytes in human peripheral blood was studied. In the dynamic observation of lymphocytes in the peripheral blood began to appear in the division phase within 36 hours, reached the first peak in 40 hours, the splitting index was 7.2%, and all the dividing cells were in the M_1 phase. . At 52 hours, the M 2 cells reached 51.3%, and the M 3 cell phase was 0.3%. In the 40-hour lymphocyte culture, the dividing phase in 10 cases was 9.08%, and the middle phase in the dividing phase was 74.0%. All of them were M1 cell phases, and thus the quality was completely satisfactory. Therefore, the 40-hour culture of human peripheral blood lymphocytes was assayed. A better method of chromosome aberrations.