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目的:观察白藜芦醇在预防阿霉素心脏毒性作用的效果及机制。方法:将30只成年雄性SD大鼠随机分为正常对照组,阿霉素模型组(单纯阿霉素干预),白藜芦醇干预组(白藜芦醇+阿霉素干预)。2周后经有创心导管监测血流动力学评估心功能,利用实时定量PCR检测心肌组织钙调节相关蛋白兰尼碱受体(RyR2)、肌浆网钙泵(SERCA2a)的mRNA水平。结果:与正常对照组相比,阿霉素模型组、白藜芦醇干预组心功能均明显下降,左室舒张压(LVDP)、左室最大收缩速率(±dP/dtmax)减小(P<0.05),左室舒张末压(LVEDP)增大(P<0.05);白藜芦醇干预组较阿霉素模型组心功能显著改善,与阿霉素模型组比较,LVDP、±dP/dtmax增大(P<0.05),LVEDP减小(P<0.05)。与正常对照组相比,阿霉素模型组、白藜芦醇干预组心肌组织中RyR2、SERCA2a的mRNA表达水平均显著下降(P<0.05),与阿霉素模型组相比,白藜芦醇干预组RyR2、SERCA2a的mRNA水平均升高(P<0.05)。结论:白藜芦醇可减轻阿霉素心脏毒性作用,保护心脏功能,其机制与改善心肌细胞钙循环调节有关。
Objective: To observe the effect and mechanism of resveratrol in preventing doxorubicin cardiotoxicity. Methods: Thirty adult male Sprague-Dawley rats were randomly divided into normal control group, doxorubicin model group (simple doxorubicin intervention) and resveratrol intervention group (resveratrol plus doxorubicin intervention). After 2 weeks, the hemodynamic assessment of cardiac function was performed by invasive catheterization. Real-time quantitative PCR was used to detect mRNA level of RyR2 and SERCA2a in cardiac muscle tissue. Results: Compared with the normal control group, the cardiac function of adriamycin group and resveratrol group were significantly decreased, and the left ventricular diastolic pressure (LVDP) and left ventricular maximal contraction rate (± dP / dtmax) decreased (P (P <0.05). Compared with doxorubicin group, the cardiac function of resveratrol intervention group was significantly improved compared with that of doxorubicin group. LVDP, ± dP / dtmax increased (P <0.05), LVEDP decreased (P <0.05). Compared with the normal control group, mRNA expression levels of RyR2 and SERCA2a in the adriamycin group and the resveratrol intervention group were significantly decreased (P <0.05). Compared with the adriamycin model group, The mRNA levels of RyR2 and SERCA2a in alcohol intervention group were significantly increased (P <0.05). Conclusion: Resveratrol can attenuate cardiotoxicity of adriamycin and protect cardiac function, and its mechanism is related to the regulation of calcium circulation in cardiomyocytes.