Cloned calves produced by nuclear transfer from cultured cumulus cells

来源 :中国科学C辑 | 被引量 : 0次 | 上传用户:my_wenzi
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
Short-term cultured cumulus cell lines (1-5BCC) derived from 5 individual cows were used in nuclear transfer (NT) and 1188 enucleated bovine oocytes matured in vitro were used as nuclear recipients. A total of 931 (78.4%) cloned embryos were reconstructed, of which 763 (82%) cleaved, 627 (67.3%) developed to 8-cell stage, and 275 (29.5%) reached blastocyst stage. The average cell number of blastocysts was 124±24.5 (n=20). In this study, the effects of donor cell sources, serum starvation of donor cells, time interval from fusion to activation (IFA) were also tested on cloning efficiency. These results showed that blastocyst rates of embryos reconstructed from 5 different individuals cells were significantly different among them (14.1%, 45.2%, 27.3%, 34.3%, vs 1.5%, P0.05); and that blastocyst rate (20.3%) of the group with fusion/activation interval of 2-3 h, was significantly lower than that of the 3-6 h groups (31.0%), while not significantly different among 3-4 h (P < 0.05), 4-5 h, and 5-6 h groups (P ≥ 0.05). Sixty-three thawed NT blastocysts were transferred to 31 recipient cows, of which 4 pregnancies were established and two cloned calves were given birth. These results indicate that serum starvation of cumulus cells is not a key factor for successful bovine cloning, while IFA treatment and sources of donor cells have effects on cloning efficiency.
其他文献
深圳市螺岭外国语实验学校,是深圳教育的窗口学校。学校成立于1981年,原名深圳市第三小学,后改名为螺岭小学。1997年被评为广东省一级学校;2002年,按照罗湖区委区政府为社会
目的:探讨如何有效规避精神病患者住院期间的暴力攻击行为.方法:采用回顾性方法分析入组病例发生暴力行为的原因、攻击对象及攻击方式.结果:暴力行为发生的原因有妄想支配、
2011年。农田水利问题被提升到“中央一号”文件的高度,标志着解决水资源短缺问题已迫在眉睫。随着农业产业结构的调整,拓宽高效节水农业路子成为农业生产的大课题。这促使化肥
胰十二指肠切除术是外科最大手术之一,复杂且刨伤大,主要适用于壶腹癌、胰头癌和十二指肠恶性肿瘤等疾病.此手术步骤复杂,手术时间长,术中出血较多、术后并发症多,病死率高.
Objective: To evaluate the safety, feasibility and short-term efficacy of early post-operational intraperitoneal chemotherapy (EPIC) combined with Salviae milti
园林绿化景观设计,是建设生态型宜居社区环境的新兴学科;是提高社区文化品位的重要艺术手段。社区园林绿化景观的设计,应坚持科学发展、以人为本的理念,遵循地域性、生态型、
Bovine foamy virus encodes a transcriptional transactivitor, Tas or Borf-1, which governs the level of viral transcripts initiated by both the promoter in the l
The research of soil classification and soil grade evaluation is often based on fuzzy theory. So, the traditional method has an inevitable problem about weight
该文采用传统统计和地统计相结合的方法 ,研究了乡 (镇 )级区域土壤养分的空间变异与分区管理技术。结果表明 ,菜田土壤主要养分N、P、K、Mn、Zn、Fe等尤其是P、Mn和Fe的速效
对耐辐射球菌(Deinococcus radiodurans)发酵液制取类胡萝卜素的提取方法和发酵培养条件进行了初步研究.用有机溶剂溶解菌体细胞并萃取得到类胡萝卜素;采用正交实验对发酵培