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黄金虎(Dendranthema grandiflorum(Ramat)Kitamura)是一种观赏性强、经济价值高的菊花品种.文章以黄金虎叶片为外植体,在MS基础培养基中添加不同浓度的植物生长调节剂进行高效稳定再生体系的建立.结果表明,叶片外植体在添加3mg/L 6-BA和1.7mg/L NAA的MS培养基中的不定芽再生率高达96.7%.不定芽在添加0.1mg/L NAA的1/2MS培养基上生根粗壮,且移栽平均存活率高.
Dendranthema grandiflorum (Ramat) Kitamura is a kind of ornamental and economical chrysanthemum species.In this paper, the golden tiger leaves were used as explants, and different concentrations of plant growth regulators were added to MS basal medium for high efficiency And the establishment of a stable regeneration system.The results showed that the regeneration rate of adventitious shoots in MS medium supplemented with 3mg / L 6-BA and 1.7mg / L NAA was as high as 96.7% .Adjusting buds were treated with 0.1mg / L NAA Of 1 / 2MS medium thick sturdy, and the average survival rate of transplanting.