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[目的]测定不同周期STZ诱导的糖尿病对心肌缺血/再灌注(I/R)损伤的影响及其与心肌VEGF表达及冠脉血管密度变化的关系。[方法]阻断和开放左冠状动脉前降支建立大鼠急性心肌I/R模型。分别用TTC染色、免疫印迹分析及Morphometric分析方法,测定大鼠心肌I/R后梗死面积、VEGF表达及冠脉血管密度。[结果]STZ处理后2周,糖尿病组(2WD)心肌梗死面积比相应周期对照组(2WC)明显缩小;STZ处理后16周(16WD),梗死面积比相应对照组(16WC)增加;Morphometric分析显示,心脏的冠脉血管密度在2WD组比2WC组显著增加(28%),而16WD组比16WC组明显减少(33%);血管内皮细胞生长因子(VEGF)的表达在2WD组比2WC组显著增加(30%),但在16WD组大鼠VEGF表达比16WC组明显减少。[结论]STZ诱导糖尿病早期、晚期对心肌I/R损伤呈现相反的作用。这可能是由于早、晚期糖尿病相反的心肌VEGF表达及冠脉血管密度改变而引起的。
[Objective] To determine the effect of different periods of STZ-induced diabetes on myocardial ischemia / reperfusion (I / R) injury and its relationship with myocardial VEGF expression and coronary artery density changes. [Methods] Acute myocardial I / R model was established by blocking and opening left anterior descending coronary artery. TTC staining, Western blot analysis and Morphometric analysis were used to determine myocardial infarct size, VEGF expression and coronary artery density after I / R. [Results] The area of myocardial infarction in diabetic group (2WD) was significantly reduced 2 weeks after STZ treatment compared with control group (2WC); the infarct size was increased at 16 weeks after STZ treatment (16WC); Morphometric analysis The results showed that the density of coronary arteries in the heart increased significantly (28%) in 2WD group compared with that in 2WC group (33%) in 16WD group. The expression of vascular endothelial growth factor (VEGF) (30%), but the expression of VEGF in 16WD group was significantly lower than that in 16WC group. [Conclusion] The early and late stages of STZ-induced diabetes have the opposite effects on myocardial I / R injury. This may be due to early and late diastolic myocardial VEGF expression and coronary vascular density caused by changes.