目的了解大鼠角膜碱烧伤后角膜干细胞的活性。方法对大鼠角膜中央区、上半周及全周3个不同区域分别进行碱性烧灼，于伤后24小时取材做离体角膜3H－Tdr掺入，计算角膜基底细胞标记率。结果中央区及上半周烧伤组角膜基底细胞标记率分别为6．784和6．002，与对照组1．29相比，H＝30．55，P＜0．05，有显著性差异，而全周烧伤组未见标记细胞。结论未完全伤及角膜缘部的碱烧伤，角膜干细胞活性增加，对角膜是否新生血管化有重要意义。 Objective To understand the activity of corneal stem cells after corneal alkali burn in rats. Methods Alkaline cauterization was performed on the central cornea, the first half of the rat and the third week of the rat cornea respectively. 3H-Tdr incorporation was performed 24 hours after the injury to calculate the corneal basal cell labeling rate. Results In the central area and the first half of the burn group, the corneal basal cell labeling rates were 6.784 and 6.002, respectively, which were significantly different from those of the control group 1.29 (H = 30.55, P <0.05) There was no marked cells in the whole burn group. Conclusion The injury of corneal limbal alkali and the increase of corneal stem cell activity are not completely injured, which is of great significance to corneal neovascularization.