应用~(32)P示踪研究磷的代谢与苹果花芽分化的关系

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测定了苹果花芽分化期间磷化合物含量的变化。发现对照的磷酸醣磷下降得比遮阴处理的快,核蛋白磷的增加则较遮阴处理高。用~(32)P 进行示踪试验看出,在对照和遮阴处理之间,~(32)P 的转化也有明显差别。~(32)P参入磷酸醣的量较遮阴处理低,而参入醣磷脂、磷脂和核蛋白的~(32)P 则较遮阴处理高。这些结果表明,遮阴处理可能由于养分供应不足,磷酸醣磷的转化受阻,以及磷脂、醣磷脂和核蛋白的合成速度下降,使正常花芽分化活动所需的磷化合物受到抑制,以致生长受阻,正常代谢作用不能进行,因而短枝花芽不能形成. The changes of phosphorus compounds during apple flower bud differentiation were measured. It was found that the control sugar and phosphate decreased more quickly than shading treatment, and the increase of nuclear protein phosphorus was higher than that of shading treatment. Tracer experiments with ~ (32) P showed that there was a significant difference in the conversion of ~ (32) P between control and shading treatments. The amount of phosphate incorporation in ~ (32) P was lower than that in shading treatment, whereas ~ (32) P, which was involved in phospholipid, phospholipid and nucleoprotein, was higher than shading treatment. These results indicate that shading may be due to insufficient supply of nutrients, inhibition of the conversion of sugar and phosphorus phosphate, and the decrease of the synthesis rate of phospholipids, glycoconjugates and nucleoproteins, the inhibition of the phosphorus compounds required for normal flower bud differentiation activities, Normal metabolism can not be carried out, and therefore spur flower buds can not form.
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