论文部分内容阅读
多位点序列分型(MLST)是近年来发展较快的以核苷酸序列分析为基础的病原菌分型的分子生物学方法,具有较高的分辨能力。通过对6～7个管家基因中长度约为470bp的核心片段的核苷序列的分析,MLST技术已广泛应用于感染性疾病病原微生物的分型鉴定,不同抗生素抵抗株及其毒力或其抗原相关性特殊基因型以及新的变异株引起的疾病流行等微生物流行病学分析。笔者下面就MLST的理论及其应用作一综述。
Multilocus sequence typing (MLST) is a molecular biological method that has been developed rapidly in recent years to classify pathogenic bacteria based on nucleotide sequence analysis and has high resolving power. MLST technique has been widely used to identify the pathogenic microorganisms of infectious diseases by analyzing the nucleotide sequences of the core fragments of about 470bp in 6 to 7 housekeeping genes. Different antibiotic resistant strains and their virulence or antigen Related genotypes and epidemics caused by new variants such as microbial epidemiology. The author of the following MLST theory and its application to make a review.