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采用紫外线遗传失活的太平洋牡蛎精子作激活源,经6-DMAP加倍诱导栉孔扇贝第二极体抑制型雌核发育二倍体;运用石蜡切片显微技术,对雌核发育二倍体卵子早期胚胎发育过程中的核相变化进行观察;采用荧光原位杂交(GISH)技术对担轮幼虫期胚胎进行检测。结果显示,紫外线处理过的精子入卵后发生一次轻微膨胀,形成雄性原核,但不形成染色体,而是浓缩为致密的染色质小体(DCB),DCB或滞留于两卵裂球的分裂沟上或进入其一的细胞质中,不与雌原核融合;GISH检测结果显示,在早期胚胎中没有检测到外源太平洋牡蛎精子的遗传物质。实验结果可为研究异精诱导栉孔扇贝雌核发育二倍体提供细胞学依据。
UV-induced genetic inactivation of Pacific oyster sperm as an activation source, doubled by 6-DMAP induced dwarfism of the second polar body inhibitory gynogenetic diploid diploid; using paraffin section microscopy, the development of gynogenetic diploid eggs Nuclear phase changes during early embryo development were observed. Fluorescent in situ hybridization (GISH) was used to detect the larval stage embryos. The results showed that the UV-treated spermatozoon slightly inflated after ovipositing to form male pronuclei, but did not form chromosomes, but concentrated to compact chromatin bodies (DCB), DCB or the cleavage ditch Or into one of the cytoplasm, not fused with the progestin; GISH test results show that in the early embryo, no foreign body oyster sperm genetic material detected. The results provide cytological evidence for the study of heterotrophic induction of gynogenetic diploid in Chlamys farreri.