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目的探讨103钯(Pd)放射性支架释放的γ射线对Fas基因表达的影响以及与胆管癌细胞凋亡的关系及意义。方法建立人胆管癌裸鼠移植瘤模型;将建模成功的裸鼠分为实验组和对照组,实验组置入37MBq103Pd胆道放射性支架,对照组置入普通金属支架;支架置入后10d计算肿瘤体积;应用TUNEL法检测胆管癌细胞凋亡的情况;免疫组化染色方法检测胆管癌细胞中Fas基因表达的情况。结果实验组肿瘤体积较对照组增长明显缓慢(P<0.05);胆管癌细胞的Fas基因表达阳性率较对照组明显增高(P<0.05),且胆管癌细胞出现明显凋亡,细胞凋亡率明显高于对照组(P<0.01)。结论103Pd放射性支架可诱导人胆管癌裸鼠移植瘤中胆管癌细胞凋亡,明显抑制胆管癌细胞生长,其可能通过增强Fas基因表达促进胆管癌细胞凋亡,可能是103Pd放射性支架治疗胆管癌的重要机理之一,进一步为临床应用103Pd放射性支架治疗胆管癌提供了理论依据。
Objective To investigate the effect of γ-ray released from 103 Pd (Palladium) radioactive stent on the expression of Fas gene and its relationship with the apoptosis of cholangiocarcinoma cells. Methods The nude mice model of human cholangiocarcinoma was established. The nude mice were divided into experimental group and control group. The experimental group received 37MBq103Pd biliary radioactive stent. The control group received ordinary metal stent. The tumor was counted 10 days after stent implantation The apoptosis of cholangiocarcinoma cells was detected by TUNEL method. The expression of Fas gene in cholangiocarcinoma cells was detected by immunohistochemical staining. Results The tumor volume in the experimental group was significantly slower than that in the control group (P <0.05). The positive rate of Fas gene expression in cholangiocarcinoma cells was significantly higher than that in the control group (P <0.05), and the apoptosis rate was significantly higher in the cholangiocarcinoma cells Obviously higher than the control group (P <0.01). Conclusion The 103Pd radioactive stent can induce the apoptosis of human cholangiocarcinoma in human bile duct carcinoma xenografts and significantly inhibit the growth of cholangiocarcinoma cells. It may promote the apoptosis of cholangiocarcinoma cells by increasing the expression of Fas gene, which may be due to the 103Pd radioactive stent treatment of cholangiocarcinoma One of the important mechanisms is to further provide a theoretical basis for the clinical application of 103Pd radioactive stent in the treatment of cholangiocarcinoma.