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作者研究了几类表面活性剂对曼氏血吸虫尾蚴的作用,以确定是否能用以防止尾蚴感染。作者将表面活性剂分成4类:1.阴离子性;2.阳离子性;3.非离子性;4.酸碱兼性。先用尾蚴制动试验对上述4类表面活性剂进行筛选。方法为:在凹玻片上,于每一凹穴分别加入1ml不同浓度的表面活性剂溶液,并加入10条曼氏血吸虫尾蚴。在解剖镜下观察尾蚴活动情况。当有5条尾蚴不活动时,记下所需时间。通过筛选,选出对曼氏血吸虫尾蚴作用较佳的一类表面活性剂。然后将筛出的表面活性剂处理尾蚴后,进行小鼠感染试验,观察1.表面活性剂与尾蚴接触时间对尾蚴感染能力的影响;2.表面活性剂的浓度对尾蚴感染能力的影响;3.表面活性
The authors studied the effect of several surfactants on Schistosoma mansoni cercariae to determine if it can be used to prevent cercariae infection. The authors classify surfactants into four classes: 1. Anionic; 2. Cationic; 3. Nonionic; 4. Acid-base facultative. The first use of cercaria braking test on the above four types of surfactant screening. The method is as follows: on a concave glass slide, add 1 ml of surfactant solution with different concentrations to each cavity, and add 10 schistosoma mansoni cercariae. Observation of cercariae activity under dissecting microscope. When there are 5 cercariae inactive, note the time required. Through screening, choose a kind of surfactant which is better to Schistosoma mansoni cercariae. After the screened surfactant was treated with cercariae, mouse infection test was performed to observe the influence of the contact time of surfactant and cercariae on the infection ability of cercariae; 2. The effect of surfactant concentration on cercariae infectivity; 3 Surface activity